An Overview of the Projects for Spring 2024
Introduction: The current semester will include a continuation of previous projects as well as the introduction of new experiments which will be overviewed in this week's blog post. From the previous year the projects to be furthered include the development of a qPCR method to test temperature effects on CRISPR-Cas proteins in Deinococcus, DNA extractions for sequencing attempts, and utilizing the 7kb plasmid from Deinococcus aquaticus. New projects will include engineering other native Deinococcus plasmids, initially the 5kb found in Deinococcus ficus, and the analysis of newly sequences genomes to ideally find a target to genetically engineer Deinococcus caeni, and learn more about the structure of Deinococcus sonorensis. Below is a detailed explanation and background of each experiment and where each stands at the present time. The qPCR experiment to test the interaction of temperature on the transcriptional regulation of CRISPR-Cas proteins has be...